Hoechst 34580 (HOE 34580)
Hoechst 34580
CAS: 23555-00-2
Molecular Formula: C27H29N7
Hoechst 34580 (HOE 34580) - Names and Identifiers
| Name | Hoechst 34580 |
| Synonyms | ProaMine HOE 34580 Hoechst 34580 HOECHST 34580 Hoechst 34580 (HOE 34580) 2,50-bibenzimidazole, 20-[p-(dimethylamino)phenyl]-5-(4-methyl-1-piperazinyl)- Benzenamine, N,N-dimethyl-4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]- |
| CAS | 23555-00-2 |
Hoechst 34580 (HOE 34580) - Physico-chemical Properties
| Molecular Formula | C27H29N7 |
| Molar Mass | 451.57 |
| Melting Point | >300℃ |
| Solubility | DMSO : ≥ 47 mg/mL (104.08 mM) |
| Appearance | powder |
| Color | Yellow to green |
| Maximum wavelength(λmax) | 392nm |
| pKa | 10.97±0.10, most acidic, temperature:25 °C; 7.66 ± 0.42, most basic(at 25℃) |
| Storage Condition | 2-8°C(protect from light) |
| Use | Hoechst 34580 is a cell-permeable fluorescent dye used for DNA and nuclear staining. |
| Target | Amyloid-β |
| In vitro study | Hoechst 34580 prefers to locate near hydrophobic regions with binding affinity with the inhibition constant IC 50 of 0.86±0.05 μM for Hoechst 34580. Hoechst 34580 is a good candidate for treating the Alzheimer’s disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 °C for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose-dependent manner. And the half-maximal concentration (IC 50 ) is examined. The IC 50 is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86±0.05 μM for Hoechst 34580. |
Hoechst 34580 (HOE 34580) - Reference
| Reference Show more | 1. Thai NQ, et al. Discovery of DNA dyes Hoechst 34580 and 33342 as good candidates for inhibiting amyloid beta formation: in silico and in vitro study. J Comput Aided Mol Des. 2016 Aug;30(8):639-50. |
Hoechst 34580 (HOE 34580) - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 2.215 ml | 11.073 ml | 22.145 ml |
| 5 mM | 0.443 ml | 2.215 ml | 4.429 ml |
| 10 mM | 0.221 ml | 1.107 ml | 2.215 ml |
| 5 mM | 0.044 ml | 0.221 ml | 0.443 ml |
Last Update：2024-01-02 23:10:35
Hoechst 34580 (HOE 34580) - Reference Information
| biological activity | Hoechst 34580 (HOE 34580) is a cell-permeable blue fluorescent dye used to stain DNA and nucleus. Hoechst 34580 is also a good candidate for the treatment of Alzheimer's disease. By inhibiting the formation of amyloid beta (Aβ), its IC50 value is 0.86 μM. |
| target | TargetValue Aβ (Cell-free say) 0.86 μM |
| Target | Value |
| Aβ (Cell-free assay) | 0.86 μM |
| in vitro study | Hoechst 34580 prefers to locate near hydrophobic regions with binding affinity with the inhibition constant IC 50 of 0.86±0.05 μM for Hoechst 34580. Hoechst 34580 is a good candidate for treating the Alzheimer's disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37°C for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose-dependent manner. And the half-maximal concentration (IC 50 ) is examined. The IC 50 is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which had 0.86±0.05 μ m for Hoechst 34580. |
| biological field application | Detecting malaria infected red blood cells,polynucleotides; nucleic acid amplification; nucleic acid sequencing |
Last Update：2024-04-09 21:54:55
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Product Name: Hoechst 34580 Visit Supplier Webpage Request for quotationCAS: 23555-00-2
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Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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